Molecules

M1R(L,S228~u~p,S273~u~p,Arr,GRK,PP1,CK2)

M1R L S228 u p S273 u p Arr GRK PP1 CK2

Oxo(R)

Oxo R

Arrestin(RLP,MEK,PP2A)

Arrestin RLP MEK PP2A

MEK(Arr,ERK)

MEK Arr ERK

ERK(MEK,s~u,PP2A,PP1)

ERK MEK s u PP2A PP1

pERK(MEK,s~p,PP2A,PP1)

pERK MEK s p PP2A PP1

Oxo_EC()

Oxo_EC

PP2A(Arrestin,ERK)

PP2A Arrestin ERK

probe(site~u)

probe site u

phos_probe(Site0~p)

phos_probe Site0 p

GRK(RL)

GRK RL

PP1(RL)

PP1 RL

CK2(RL)

CK2 RL

Species

Oxo_EC()

Oxo_EC

M1R(L,S228~u,S273~u,Arr,GRK,PP1,CK2)

M1R L S228 u S273 u Arr GRK PP1 CK2

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK,PP1,CK2)

Oxo 1R M1R 1L S228 u S273 u Arr GRK PP1 CK2

Arrestin(RLP,MEK,PP2A)

Arrestin RLP MEK PP2A

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP MEK PP2A

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP 3MEK PP2A MEK 3Arr ERK

ERK(MEK,s~u,PP2A,PP1)

ERK MEK s u PP2A PP1

MEK(Arr,ERK)

MEK Arr ERK

PP2A(Arrestin,ERK)

PP2A Arrestin ERK

pERK(MEK,s~p,PP2A,PP1)

pERK MEK s p PP2A PP1

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK!4).ERK(MEK!4,s~u,PP2A,PP1)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP 3MEK PP2A MEK 3Arr 4ERK ERK 4MEK s u PP2A PP1

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK!4).pERK(MEK!4,s~p,PP2A,PP1)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP 3MEK PP2A MEK 3Arr 4ERK pERK 4MEK s p PP2A PP1

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A)

Oxo 1R M1R 1L S228 u S273 u 2Arr GRK PP1 CK2 Arrestin 2RLP MEK PP2A

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A!3).PP2A(Arrestin!3,ERK)

Oxo 1R M1R 1L S228 u S273 u 2Arr GRK PP1 CK2 Arrestin 2RLP MEK 3PP2A PP2A 3Arrestin ERK

probe(site~u)

probe site u

phos_probe(Site0~p)

phos_probe Site0 p

GRK(RL)

GRK RL

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK!2,PP1,CK2).GRK(RL!2)

Oxo 1R M1R 1L S228 u S273 u Arr 2GRK PP1 CK2 GRK 2RL

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK!2,PP1,CK2).GRK(RL!2)

Oxo 1R M1R 1L S228 p S273 p Arr 2GRK PP1 CK2 GRK 2RL

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK,PP1,CK2)

Oxo 1R M1R 1L S228 p S273 p Arr GRK PP1 CK2

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A!3).PP2A(Arrestin!3,ERK!4).pERK(MEK,s~p,PP2A!4,PP1)

Oxo 1R M1R 1L S228 u S273 u 2Arr GRK PP1 CK2 Arrestin 2RLP MEK 3PP2A PP2A 3Arrestin 4ERK pERK MEK s p 4PP2A PP1

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A!3).PP2A(Arrestin!3,ERK!4).ERK(MEK,s~u,PP2A!4,PP1)

Oxo 1R M1R 1L S228 u S273 u 2Arr GRK PP1 CK2 Arrestin 2RLP MEK 3PP2A PP2A 3Arrestin 4ERK ERK MEK s u 4PP2A PP1

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK,PP1!2,CK2).PP1(RL!2)

Oxo 1R M1R 1L S228 u S273 u Arr GRK 2PP1 CK2 PP1 2RL

PP1(RL)

PP1 RL

CK2(RL)

CK2 RL

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK,PP1,CK2!2).CK2(RL!2)

Oxo 1R M1R 1L S228 u S273 u Arr GRK PP1 2CK2 CK2 2RL

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK,PP1,CK2!2).CK2(RL!2)

Oxo 1R M1R 1L S228 p S273 p Arr GRK PP1 2CK2 CK2 2RL

Observables

M1R(L,S228,S273,Arr,GRK,PP1,CK2)

M1R L S228 S273 Arr GRK PP1 CK2

Oxo(R)

Oxo R

Arrestin(RLP,MEK,PP2A)

Arrestin RLP MEK PP2A

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP MEK PP2A

MEK(Arr,ERK)

MEK Arr ERK

ERK(MEK,s~u,PP2A,PP1)

ERK MEK s u PP2A PP1

pERK(MEK,s~p,PP2A,PP1)

pERK MEK s p PP2A PP1

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP 3MEK PP2A MEK 3Arr ERK

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK,PP1,CK2)

Oxo 1R M1R 1L S228 p S273 p Arr GRK PP1 CK2

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A)

Oxo 1R M1R 1L S228 u S273 u 2Arr GRK PP1 CK2 Arrestin 2RLP MEK PP2A

Oxo_EC()

Oxo_EC

PP2A(Arrestin,ERK)

PP2A Arrestin ERK

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A!3).PP2A(Arrestin!3,ERK)

Oxo 1R M1R 1L S228 u S273 u 2Arr GRK PP1 CK2 Arrestin 2RLP MEK 3PP2A PP2A 3Arrestin ERK

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK!4).ERK(MEK!4,s~u)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP 3MEK PP2A MEK 3Arr 4ERK ERK 4MEK s u PP2A PP1

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK!4).pERK(MEK!4,s~p)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP 3MEK PP2A MEK 3Arr 4ERK pERK 4MEK s p PP2A PP1

probe()

probe site u

phos_probe()

phos_probe Site0 p

GRK()

GRK RL

PP1()

PP1 RL

CK2()

CK2 RL

Reaction Rules

Oxo(R) + M1R(L,S228~u,S273~u,Arr,GRK,PP1,CK2) <-> Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK,PP1,CK2)

Oxo 1 ⬆⬇R M1R 1 ⬆⬇L S228 u S273 u Arr GRK PP1 CK2

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK,PP1,CK2) + Arrestin(RLP,MEK,PP2A) <-> Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A)

Oxo 1R M1R 1L S228 p S273 p 2 ⬆⬇Arr GRK PP1 CK2 Arrestin 2 ⬆⬇RLP MEK PP2A

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK,PP1,CK2) + Arrestin(RLP,MEK,PP2A) <-> Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A)

Oxo 1R M1R 1L S228 u S273 u 2 ⬆⬇Arr GRK PP1 CK2 Arrestin 2 ⬆⬇RLP MEK PP2A

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK,PP1,CK2) -> Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK,PP1,CK2)

Oxo 1R M1R 1L S228 u p take one or more branches, once each, in any order⬆⬆ S273 u p take one or more branches, once each, in any order⬆⬆ Arr GRK PP1 CK2

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK!2,PP1,CK2).GRK(RL!2) -> Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK!2,PP1,CK2).GRK(RL!2)

Oxo 1R M1R 1L S228 u p take all branches, once each, in any order⬇⬇ S273 u p take all branches, once each, in any order⬇⬇ Arr 2GRK PP1 CK2 GRK 2RL

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A) + MEK(Arr,ERK) <-> Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP 3 ⬆⬇MEK PP2A MEK 3 ⬆⬇Arr ERK

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK) + ERK(MEK,s~u,PP2A,PP1) <-> Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK!4).ERK(MEK!4,s~u,PP2A,PP1)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP 3MEK PP2A MEK 3Arr 4 ⬆⬇ERK ERK 4 ⬆⬇MEK s u PP2A PP1

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A) + PP2A(Arrestin,ERK) <-> Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK,PP2A!3).PP2A(Arrestin!3,ERK)

Oxo 1R M1R 1L S228 u S273 u 2Arr GRK PP1 CK2 Arrestin 2RLP MEK 3 ⬆⬇PP2A PP2A 3 ⬆⬇Arrestin ERK

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK!4).pERK(MEK!4,s~p,PP2A,PP1) <-> Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr!2,GRK,PP1,CK2).Arrestin(RLP!2,MEK!3,PP2A).MEK(Arr!3,ERK) + pERK(MEK,s~p,PP2A,PP1)

Oxo 1R M1R 1L S228 p S273 p 2Arr GRK PP1 CK2 Arrestin 2RLP 3MEK PP2A MEK 3Arr 4 ⬆⬇ERK pERK 4 ⬆⬇MEK s p PP2A PP1

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK,PP1,CK2) + GRK(RL) <-> Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK!2,PP1,CK2).GRK(RL!2)

Oxo 1R M1R 1L S228 u S273 u Arr 2 ⬆⬇GRK PP1 CK2 GRK 2 ⬆⬇RL

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK!2,PP1,CK2).GRK(RL!2) <-> Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK,PP1,CK2) + GRK(RL)

Oxo 1R M1R 1L S228 p S273 p Arr 2 ⬆⬇GRK PP1 CK2 GRK 2 ⬆⬇RL

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK,PP1,CK2) + CK2(RL) <-> Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK,PP1,CK2!2).CK2(RL!2)

Oxo 1R M1R 1L S228 u S273 u Arr GRK PP1 2 ⬆⬇CK2 CK2 2 ⬆⬇RL

Oxo(R!1).M1R(L!1,S228~u,S273~u,Arr,GRK,PP1,CK2!2).CK2(RL!2) -> Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK,PP1,CK2!2).CK2(RL!2)

Oxo 1R M1R 1L S228 u p take all branches, once each, in any order⬇⬇ S273 u p take all branches, once each, in any order⬇⬇ Arr GRK PP1 2CK2 CK2 2RL

Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK,PP1,CK2!2).CK2(RL!2) <-> Oxo(R!1).M1R(L!1,S228~p,S273~p,Arr,GRK,PP1,CK2) + CK2(RL)

Oxo 1R M1R 1L S228 p S273 p Arr GRK PP1 2 ⬆⬇CK2 CK2 2 ⬆⬇RL