Molecules

Descriptions of molecule types can be found in the Supplementary Text (model guide, File S3)

Lig1(aCD28,aCD28)

Lig1 aCD28 aCD28

Lig2(aCD28,aCD3)

Lig2 aCD28 aCD3

Lig3(aCD3,aCD3)

Lig3 aCD3 aCD3

TCR(epitope,Y149_D~0~P,Y171_G~0~P,Y111~0~P,Y123~0~P,fynbind,PRS_E,Y188_E~0~P,Y199_E~0~P)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

CD28(epitope,PRS1,PRS2)

CD28 epitope PRS1 PRS2

LCK(SH2,SH3,Y192~0~P,Y424~0~P,Y505~0~P)

LCK SH2 SH3 Y192 0 P Y424 0 P Y505 0 P

ITK(SH3,SH2,PTK,Y512~0~P)

ITK SH3 SH2 PTK Y512 0 P

ZAP70(SH2,PTK,Y493~0~P)

ZAP70 SH2 PTK Y493 0 P

PTPN6(SH2,PTP,Y566~0~P)

PTPN6 SH2 PTP Y566 0 P

PAG1(Y163~0~P,Y317~0~P)

PAG1 Y163 0 P Y317 0 P

CSK(SH2)

CSK SH2

DOK1(Y449~0~P)

DOK1 Y449 0 P

DOK2(Y299~0~P)

DOK2 Y299 0 P

FYN(unique,PTK)

FYN unique PTK

NCK(SH3_1,SH3_3,SH2)

NCK SH3_1 SH3_3 SH2

WAS(PRS,Y291~0~P)

WAS PRS Y291 0 P

LAT(Y132~0~P,Y191~0~P)

LAT Y132 0 P Y191 0 P

PLCG1(SH2,SH3,Y783~0~P)

PLCG1 SH2 SH3 Y783 0 P

GRAP2(SH2,SH3)

GRAP2 SH2 SH3

LCP2(RxxK,Y113_Y128~0~P,PRS,Y145~0~P)

LCP2 RxxK Y113_Y128 0 P PRS Y145 0 P

Species

Seed species are used to initialize a simulation. Following each molecule is parameter that specifies copy number

Lig1(aCD28,aCD28)

Lig1 aCD28 aCD28

Lig2(aCD28,aCD3)

Lig2 aCD28 aCD3

Lig3(aCD3,aCD3)

Lig3 aCD3 aCD3

CD28(epitope,PRS1,PRS2)

CD28 epitope PRS1 PRS2

TCR(epitope,Y149_D~0,Y171_G~0,Y111~0,Y123~0,fynbind,PRS_E,Y188_E~0,Y199_E~0)

TCR epitope Y149_D 0 Y171_G 0 Y111 0 Y123 0 fynbind PRS_E Y188_E 0 Y199_E 0

LCK(SH2,SH3,Y192~0,Y424~0,Y505~0)

LCK SH2 SH3 Y192 0 Y424 0 Y505 0

ITK(SH3,SH2,PTK,Y512~0)

ITK SH3 SH2 PTK Y512 0

ZAP70(SH2,PTK,Y493~0)

ZAP70 SH2 PTK Y493 0

PAG1(Y163~0,Y317~0)

PAG1 Y163 0 Y317 0

CSK(SH2)

CSK SH2

DOK1(Y449~0)

DOK1 Y449 0

DOK2(Y299~0)

DOK2 Y299 0

FYN(unique,PTK)

FYN unique PTK

NCK(SH3_1,SH3_3,SH2)

NCK SH3_1 SH3_3 SH2

WAS(PRS,Y291~0)

WAS PRS Y291 0

LAT(Y132~0,Y191~0)

LAT Y132 0 Y191 0

PLCG1(SH2,SH3,Y783~0)

PLCG1 SH2 SH3 Y783 0

GRAP2(SH2,SH3)

GRAP2 SH2 SH3
To simulate PTPN6 knockdown, the copy number of PTPN6 was set to 0.

PTPN6(SH2,PTP,Y566~0)

PTPN6 SH2 PTP Y566 0
To simulate LCP2 knockdown, the copy number of LCP2 was set to 0.

LCP2(RxxK,Y113_Y128~0,PRS,Y145~0)

LCP2 RxxK Y113_Y128 0 PRS Y145 0

Observables

Beside each observable is noted the figure(s) in which the observable is plotted.

TCR(Y149_D~P!?)

TCR epitope Y149_D P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y171_G~P!?)

TCR epitope Y149_D 0 P Y171_G P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y111~P!?)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y123~P!?)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y188_E~P!?)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E P Y199_E 0 P

TCR(Y199_E~P!?)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E P

ZAP70(Y493~P!?)

ZAP70 SH2 PTK Y493 P

LCK(Y424~P!?)

LCK SH2 SH3 Y192 0 P Y424 P Y505 0 P

LCK(Y192~P!?)

LCK SH2 SH3 Y192 P Y424 0 P Y505 0 P

ITK(Y512~P!?)

ITK SH3 SH2 PTK Y512 P

PTPN6(Y566~P!?)

PTPN6 SH2 PTP Y566 P

WAS(Y291~P!?)

WAS PRS Y291 P

PAG1(Y163~P!?)

PAG1 Y163 P Y317 0 P

DOK2(Y299~P!?)

DOK2 Y299 P

DOK1(Y449~P!?)

DOK1 Y449 P

PLCG1(Y783~P!?)

PLCG1 SH2 SH3 Y783 P

LAT(Y191~P!?)

LAT Y132 0 P Y191 P

LCK(Y505~P!?)

LCK SH2 SH3 Y192 0 P Y424 0 P Y505 P

TCR(PRS_E!1).NCK(SH3_1!1)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind 1PRS_E Y188_E 0 P Y199_E 0 P NCK 1SH3_1 SH3_3 SH2

LCP2(Y113_Y128~P!3).NCK(SH2!3)

LCP2 RxxK Y113_Y128 3P PRS Y145 0 P NCK SH3_1 SH3_3 3SH2

Reaction Rules

Rules are numbered to correspond to entries in Supplementary Text (model guide), where rules are annotatedRule 1a

Lig1(aCD28,aCD28) + CD28(epitope) <-> Lig1(aCD28!1,aCD28).CD28(epitope!1)

Lig1 1 ⬆⬇aCD28 aCD28 CD28 1 ⬆⬇epitope PRS1 PRS2
Rule 1b

Lig1(aCD28!1,aCD28).CD28(epitope!1) + CD28(epitope) <-> Lig1(aCD28!1,aCD28!2).CD28(epitope!1).CD28(epitope!2)

Lig1 1aCD28 2 ⬆⬇aCD28 CD28 1epitope PRS1 PRS2 CD28 2 ⬆⬇epitope PRS1 PRS2
Rule 2a

Lig3(aCD3,aCD3) + TCR(epitope) <-> Lig3(aCD3!1,aCD3).TCR(epitope!1)

Lig3 1 ⬆⬇aCD3 aCD3 TCR 1 ⬆⬇epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 2b

Lig3(aCD3!1,aCD3).TCR(epitope!1) + TCR(epitope) <-> Lig3(aCD3!1,aCD3!2).TCR(epitope!1).TCR(epitope!2)

Lig3 1aCD3 2 ⬆⬇aCD3 TCR 1epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P TCR 2 ⬆⬇epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 3a

Lig2(aCD28,aCD3) + CD28(epitope) <-> Lig2(aCD28!1,aCD3).CD28(epitope!1)

Lig2 1 ⬆⬇aCD28 aCD3 CD28 1 ⬆⬇epitope PRS1 PRS2
Rule 3b

Lig2(aCD28!1,aCD3).CD28(epitope!1) + TCR(epitope) <-> Lig2(aCD28!1,aCD3!2).CD28(epitope!1).TCR(epitope!2)

Lig2 1aCD28 2 ⬆⬇aCD3 CD28 1epitope PRS1 PRS2 TCR 2 ⬆⬇epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 3c

Lig2(aCD28,aCD3) + TCR(epitope) <-> Lig2(aCD28,aCD3!1).TCR(epitope!1)

Lig2 aCD28 1 ⬆⬇aCD3 TCR 1 ⬆⬇epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 3d

Lig2(aCD28,aCD3!1).TCR(epitope!1) + CD28(epitope) <-> Lig2(aCD28!2,aCD3!1).TCR(epitope!1).CD28(epitope!2)

Lig2 2 ⬆⬇aCD28 1aCD3 TCR 1epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P CD28 2 ⬆⬇epitope PRS1 PRS2
Rule 4

LCK(SH3) + CD28(PRS1) <-> LCK(SH3!1).CD28(PRS1!1)

LCK SH2 1 ⬆⬇SH3 Y192 0 P Y424 0 P Y505 0 P CD28 epitope 1 ⬆⬇PRS1 PRS2
Rule 5

CD28(PRS2) + ITK(SH3) <-> CD28(PRS2!1).ITK(SH3!1)

CD28 epitope PRS1 1 ⬆⬇PRS2 ITK 1 ⬆⬇SH3 SH2 PTK Y512 0 P
Rule 6

TCR(fynbind) + FYN(unique) <-> TCR(fynbind!1).FYN(unique!1)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P 1 ⬆⬇fynbind PRS_E Y188_E 0 P Y199_E 0 P FYN 1 ⬆⬇unique PTK
Rule 7a

NCK(SH3_1) + TCR(Y188_E~0,PRS_E) <-> NCK(SH3_1!1).TCR(Y188_E~0,PRS_E!1)

NCK 1 ⬆⬇SH3_1 SH3_3 SH2 TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind 1 ⬆⬇PRS_E Y188_E 0 Y199_E 0 P
Rule 7b

NCK(SH3_1!1).TCR(Y188_E~P,PRS_E!1) -> NCK(SH3_1) + TCR(Y188_E~P,PRS_E)

NCK 1 ⬆SH3_1 SH3_3 SH2 TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind 1 ⬆PRS_E Y188_E P Y199_E 0 P
Rule 8

TCR(Y111~P) + ZAP70(SH2) <-> TCR(Y111~P!1).ZAP70(SH2!1)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 1 ⬆⬇P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P ZAP70 1 ⬆⬇SH2 PTK Y493 0 P
Rule 9

TCR(Y123~P) + ZAP70(SH2) <-> TCR(Y123~P!1).ZAP70(SH2!1)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 1 ⬆⬇P fynbind PRS_E Y188_E 0 P Y199_E 0 P ZAP70 1 ⬆⬇SH2 PTK Y493 0 P
Rule 10

TCR(Y199_E~P) + ZAP70(SH2) <-> TCR(Y199_E~P!1).ZAP70(SH2!1)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 1 ⬆⬇P ZAP70 1 ⬆⬇SH2 PTK Y493 0 P
Rule 11

TCR(Y188_E~P) + ZAP70(SH2) <-> TCR(Y188_E~P!1).ZAP70(SH2!1)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 1 ⬆⬇P Y199_E 0 P ZAP70 1 ⬆⬇SH2 PTK Y493 0 P
Rule 12

PTPN6(SH2) + TCR(Y149_D~P) <-> PTPN6(SH2!1).TCR(Y149_D~P!1)

PTPN6 1 ⬆⬇SH2 PTP Y566 0 P TCR epitope Y149_D 1 ⬆⬇P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 13

PTPN6(SH2) + TCR(Y171_G~P) <-> PTPN6(SH2!1).TCR(Y171_G~P!1)

PTPN6 1 ⬆⬇SH2 PTP Y566 0 P TCR epitope Y149_D 0 P Y171_G 1 ⬆⬇P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 14

LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y188_E~0) -> LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y188_E~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 0 CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P take all branches, once each, in any order⬇⬇ Y199_E 0 P
Rule 15

LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y199_E~0) -> LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y199_E~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 0 CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P take all branches, once each, in any order⬇⬇
Rule 16

LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y149_D~0) -> LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y149_D~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 0 CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 0 P take all branches, once each, in any order⬇⬇ Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 17

LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y171_G~0) -> LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y171_G~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 0 CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 0 P Y171_G 0 P take all branches, once each, in any order⬇⬇ Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 18

LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y111~0) -> LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y111~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 0 CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 0 P Y171_G 0 P Y111 0 P take all branches, once each, in any order⬇⬇ Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 19

LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y123~0) -> LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y123~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 0 CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P take all branches, once each, in any order⬇⬇ fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 20a

LCK(SH3!1,Y505~P).CD28(PRS1!1,epitope!2).Lig1(aCD28!2,aCD28!3).CD28(epitope!3,PRS1!4).LCK(SH3!4,Y424~0) -> LCK(SH3!1,Y505~P).CD28(PRS1!1,epitope!2).Lig1(aCD28!2,aCD28!3).CD28(epitope!3,PRS1!4).LCK(SH3!4,Y424~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 P CD28 2epitope 1PRS1 PRS2 Lig1 2aCD28 3aCD28 CD28 3epitope 4PRS1 PRS2 LCK SH2 4SH3 Y192 0 P Y424 0 P take all branches, once each, in any order⬇⬇ Y505 0 P
Rule 20b

LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig1(aCD28!2,aCD28!3).CD28(epitope!3,PRS1!4).LCK(SH3!4,Y424~0) -> LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig1(aCD28!2,aCD28!3).CD28(epitope!3,PRS1!4).LCK(SH3!4,Y424~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 0 CD28 2epitope 1PRS1 PRS2 Lig1 2aCD28 3aCD28 CD28 3epitope 4PRS1 PRS2 LCK SH2 4SH3 Y192 0 P Y424 0 P take all branches, once each, in any order⬇⬇ Y505 0 P
Rule 21a

LCK(SH3!1,Y505~P).CD28(PRS1!1,epitope!2).Lig1(aCD28!2,aCD28!3).CD28(epitope!3,PRS2!4).ITK(SH3!4,Y512~0) -> LCK(SH3!1,Y505~P).CD28(PRS1!1,epitope!2).Lig1(aCD28!2,aCD28!3).CD28(epitope!3,PRS2!4).ITK(SH3!4,Y512~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 P CD28 2epitope 1PRS1 PRS2 Lig1 2aCD28 3aCD28 CD28 3epitope PRS1 4PRS2 ITK 4SH3 SH2 PTK Y512 0 P take all branches, once each, in any order⬇⬇
Rule 21b

LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig1(aCD28!2,aCD28!3).CD28(epitope!3,PRS2!4).ITK(SH3!4,Y512~0) -> LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig1(aCD28!2,aCD28!3).CD28(epitope!3,PRS2!4).ITK(SH3!4,Y512~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 0 CD28 2epitope 1PRS1 PRS2 Lig1 2aCD28 3aCD28 CD28 3epitope PRS1 4PRS2 ITK 4SH3 SH2 PTK Y512 0 P take all branches, once each, in any order⬇⬇
Rule 22a

TCR(epitope!3,Y111~P!1).ZAP70(Y493~0,SH2!1).Lig2(aCD28!2,aCD3!3).CD28(epitope!2,PRS1!4).LCK(SH3!4,Y505~0) -> TCR(epitope!3,Y111~P!1).ZAP70(Y493~P,SH2!1).Lig2(aCD28!2,aCD3!3).CD28(epitope!2,PRS1!4).LCK(SH3!4,Y505~0)

TCR 3epitope Y149_D 0 P Y171_G 0 P Y111 1P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P ZAP70 1SH2 PTK Y493 0 P take all branches, once each, in any order⬇⬇ Lig2 2aCD28 3aCD3 CD28 2epitope 4PRS1 PRS2 LCK SH2 4SH3 Y192 0 P Y424 0 P Y505 0
Rule 22b

TCR(epitope!3,Y123~P!1).ZAP70(Y493~0,SH2!1).Lig2(aCD28!2,aCD3!3).CD28(epitope!2,PRS1!4).LCK(SH3!4,Y505~0) -> TCR(epitope!3,Y123~P!1).ZAP70(Y493~P,SH2!1).Lig2(aCD28!2,aCD3!3).CD28(epitope!2,PRS1!4).LCK(SH3!4,Y505~0)

TCR 3epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 1P fynbind PRS_E Y188_E 0 P Y199_E 0 P ZAP70 1SH2 PTK Y493 0 P take all branches, once each, in any order⬇⬇ Lig2 2aCD28 3aCD3 CD28 2epitope 4PRS1 PRS2 LCK SH2 4SH3 Y192 0 P Y424 0 P Y505 0
Rule 22c

TCR(epitope!3,Y188_E~P!1).ZAP70(Y493~0,SH2!1).Lig2(aCD28!2,aCD3!3).CD28(epitope!2,PRS1!4).LCK(SH3!4,Y505~0) -> TCR(epitope!3,Y188_E~P!1).ZAP70(Y493~P,SH2!1).Lig2(aCD28!2,aCD3!3).CD28(epitope!2,PRS1!4).LCK(SH3!4,Y505~0)

TCR 3epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 1P Y199_E 0 P ZAP70 1SH2 PTK Y493 0 P take all branches, once each, in any order⬇⬇ Lig2 2aCD28 3aCD3 CD28 2epitope 4PRS1 PRS2 LCK SH2 4SH3 Y192 0 P Y424 0 P Y505 0
Rule 22d

TCR(epitope!3,Y199_E~P!1).ZAP70(Y493~0,SH2!1).Lig2(aCD28!2,aCD3!3).CD28(epitope!2,PRS1!4).LCK(SH3!4,Y505~0) -> TCR(epitope!3,Y199_E~P!1).ZAP70(Y493~P,SH2!1).Lig2(aCD28!2,aCD3!3).CD28(epitope!2,PRS1!4).LCK(SH3!4,Y505~0)

TCR 3epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 1P ZAP70 1SH2 PTK Y493 0 P take all branches, once each, in any order⬇⬇ Lig2 2aCD28 3aCD3 CD28 2epitope 4PRS1 PRS2 LCK SH2 4SH3 Y192 0 P Y424 0 P Y505 0
Rule 23a

LCK(SH3!1,Y505~P).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y149_D~P!4).PTPN6(SH2!4,Y566~0) -> LCK(SH3!1,Y505~P).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y149_D~P!4).PTPN6(SH2!4,Y566~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 P CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 4P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P PTPN6 4SH2 PTP Y566 0 P take all branches, once each, in any order⬇⬇
Rule 23b

LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y149_D~P!4).PTPN6(SH2!4,Y566~0) -> LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y149_D~P!4).PTPN6(SH2!4,Y566~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 0 CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 4P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P PTPN6 4SH2 PTP Y566 0 P take all branches, once each, in any order⬇⬇
Rule 23c

LCK(SH3!1,Y505~P).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y171_G~P!4).PTPN6(SH2!4,Y566~0) -> LCK(SH3!1,Y505~P).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y171_G~P!4).PTPN6(SH2!4,Y566~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 P CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 0 P Y171_G 4P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P PTPN6 4SH2 PTP Y566 0 P take all branches, once each, in any order⬇⬇
Rule 23d

LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y171_G~P!4).PTPN6(SH2!4,Y566~0) -> LCK(SH3!1,Y505~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y171_G~P!4).PTPN6(SH2!4,Y566~P)

LCK SH2 1SH3 Y192 0 P Y424 0 P Y505 0 CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 0 P Y171_G 4P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P PTPN6 4SH2 PTP Y566 0 P take all branches, once each, in any order⬇⬇
Rule 24a

LCK(SH2,Y192~0) + PTPN6(Y566~P) <-> LCK(SH2!1,Y192~0).PTPN6(Y566~P!1)

LCK 1 ⬆⬇SH2 SH3 Y192 0 Y424 0 P Y505 0 P PTPN6 SH2 PTP Y566 1 ⬆⬇P
Rule 24b

LCK(SH2,Y192~P) + PTPN6(Y566~P) <-> LCK(SH2!1,Y192~P).PTPN6(Y566~P!1)

LCK 1 ⬆⬇SH2 SH3 Y192 P Y424 0 P Y505 0 P PTPN6 SH2 PTP Y566 1 ⬆⬇P
Rule 25

PAG1(Y317~P) + CSK(SH2) <-> PAG1(Y317~P!1).CSK(SH2!1)

PAG1 Y163 0 P Y317 1 ⬆⬇P CSK 1 ⬆⬇SH2
Rule 26

PAG1(Y163~P) + LCK(SH2) <-> PAG1(Y163~P!1).LCK(SH2!1)

PAG1 Y163 1 ⬆⬇P Y317 0 P LCK 1 ⬆⬇SH2 SH3 Y192 0 P Y424 0 P Y505 0 P
Rule 27

PAG1(Y317~0,Y163~P!1).LCK(SH2!1) -> PAG1(Y317~P,Y163~P!1).LCK(SH2!1)

PAG1 Y163 1P Y317 0 P take all branches, once each, in any order⬇⬇ LCK 1SH2 SH3 Y192 0 P Y424 0 P Y505 0 P
Rule 28

PAG1(Y317~P!2,Y163~P!1).LCK(SH2!1,Y505~0).CSK(SH2!2) -> PAG1(Y317~P!2,Y163~P!1).LCK(SH2!1,Y505~P).CSK(SH2!2)

PAG1 Y163 1P Y317 2P LCK 1SH2 SH3 Y192 0 P Y424 0 P Y505 0 P take all branches, once each, in any order⬇⬇ CSK 2SH2
Rule 29a

LCK(SH3!1,Y192~P).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y149_D~P!4).PTPN6(SH2!4,Y566~P!?) -> LCK(SH3!1,Y192~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y149_D~P!4).PTPN6(SH2!4,Y566~P!?)

LCK SH2 1SH3 Y192 0 P take one or more branches, once each, in any order⬆⬆ Y424 0 P Y505 0 P CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 4P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P PTPN6 4SH2 PTP Y566 P
Rule 29b

LCK(SH3!1,Y192~P).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y171_G~P!4).PTPN6(SH2!4,Y566~P!?) -> LCK(SH3!1,Y192~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y171_G~P!4).PTPN6(SH2!4,Y566~P!?)

LCK SH2 1SH3 Y192 0 P take one or more branches, once each, in any order⬆⬆ Y424 0 P Y505 0 P CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 0 P Y171_G 4P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P PTPN6 4SH2 PTP Y566 P
Rule 30a

LCK(SH3!1,Y424~P).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y149_D~P!4).PTPN6(SH2!4,Y566~P!?) -> LCK(SH3!1,Y424~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y149_D~P!4).PTPN6(SH2!4,Y566~P!?)

LCK SH2 1SH3 Y192 0 P Y424 0 P take one or more branches, once each, in any order⬆⬆ Y505 0 P CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 4P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P PTPN6 4SH2 PTP Y566 P
Rule 30b

LCK(SH3!1,Y424~P).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y171_G~P!4).PTPN6(SH2!4,Y566~P!?) -> LCK(SH3!1,Y424~0).CD28(PRS1!1,epitope!2).Lig2(aCD28!2,aCD3!3).TCR(epitope!3,Y171_G~P!4).PTPN6(SH2!4,Y566~P!?)

LCK SH2 1SH3 Y192 0 P Y424 0 P take one or more branches, once each, in any order⬆⬆ Y505 0 P CD28 2epitope 1PRS1 PRS2 Lig2 2aCD28 3aCD3 TCR 3epitope Y149_D 0 P Y171_G 4P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P PTPN6 4SH2 PTP Y566 P
Rule 30c

PTPN6(Y566~P!1).LCK(SH2!1,Y424~P) -> PTPN6(Y566~P!1).LCK(SH2!1,Y424~0)

PTPN6 SH2 PTP Y566 1P LCK 1SH2 SH3 Y192 0 P Y424 0 P take one or more branches, once each, in any order⬆⬆ Y505 0 P
Rule 31a

PAG1(Y163~P) + PTPN6(SH2!2,PTP).TCR(Y149_D~P!2) -> PAG1(Y163~P!1).PTPN6(SH2!2,PTP!1).TCR(Y149_D~P!2)

PAG1 Y163 1 ⬇P Y317 0 P PTPN6 2SH2 1 ⬇PTP Y566 0 P TCR epitope Y149_D 2P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 31b

PAG1(Y163~P) + PTPN6(SH2!2,PTP).TCR(Y171_G~P!2) -> PAG1(Y163~P!1).PTPN6(SH2!2,PTP!1).TCR(Y171_G~P!2)

PAG1 Y163 1 ⬇P Y317 0 P PTPN6 2SH2 1 ⬇PTP Y566 0 P TCR epitope Y149_D 0 P Y171_G 2P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 31c

PTPN6(PTP,SH2) + PAG1(Y163~P) -> PTPN6(PTP!1,SH2).PAG1(Y163~P!1)

PTPN6 SH2 1 ⬇PTP Y566 0 P PAG1 Y163 1 ⬇P Y317 0 P
Rule 31d

PAG1(Y163~P!1).PTPN6(PTP!1) -> PAG1(Y163~P) + PTPN6(PTP)

PAG1 Y163 1 ⬆P Y317 0 P PTPN6 SH2 1 ⬆PTP Y566 0 P
Rule 31e

PAG1(Y163~P!1).PTPN6(PTP!1,Y566~P!?) -> PAG1(Y163~0) + PTPN6(PTP,Y566~P!?)

PAG1 Y163 0 1 ⬆P take one or more branches, once each, in any order⬆⬆ Y317 0 P PTPN6 SH2 1 ⬆PTP Y566 P
Rule 32a

DOK1(Y449~P) + PTPN6(SH2!2,PTP).TCR(Y149_D~P!2) -> DOK1(Y449~P!1).PTPN6(SH2!2,PTP!1).TCR(Y149_D~P!2)

DOK1 Y449 1 ⬇P PTPN6 2SH2 1 ⬇PTP Y566 0 P TCR epitope Y149_D 2P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 32b

DOK1(Y449~P) + PTPN6(SH2!2,PTP).TCR(Y171_G~P!2) -> DOK1(Y449~P!1).PTPN6(SH2!2,PTP!1).TCR(Y171_G~P!2)

DOK1 Y449 1 ⬇P PTPN6 2SH2 1 ⬇PTP Y566 0 P TCR epitope Y149_D 0 P Y171_G 2P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 32c

DOK1(Y449~P!1).PTPN6(PTP!1) -> DOK1(Y449~P) + PTPN6(PTP)

DOK1 Y449 1 ⬆P PTPN6 SH2 1 ⬆PTP Y566 0 P
Rule 32d

DOK1(Y449~P!1).PTPN6(PTP!1,Y566~P!?) -> DOK1(Y449~0) + PTPN6(PTP,Y566~P!?)

DOK1 Y449 0 1 ⬆P take one or more branches, once each, in any order⬆⬆ PTPN6 SH2 1 ⬆PTP Y566 P
Rule 33a

DOK2(Y299~P) + PTPN6(SH2!2,PTP).TCR(Y149_D~P!2) -> DOK2(Y299~P!1).PTPN6(SH2!2,PTP!1).TCR(Y149_D~P!2)

DOK2 Y299 1 ⬇P PTPN6 2SH2 1 ⬇PTP Y566 0 P TCR epitope Y149_D 2P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 33b

DOK2(Y299~P) + PTPN6(SH2!2,PTP).TCR(Y171_G~P!2) -> DOK2(Y299~P!1).PTPN6(SH2!2,PTP!1).TCR(Y171_G~P!2)

DOK2 Y299 1 ⬇P PTPN6 2SH2 1 ⬇PTP Y566 0 P TCR epitope Y149_D 0 P Y171_G 2P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P
Rule 33c

DOK2(Y299~P!1).PTPN6(PTP!1) -> DOK2(Y299~P) + PTPN6(PTP)

DOK2 Y299 1 ⬆P PTPN6 SH2 1 ⬆PTP Y566 0 P
Rule 33d

DOK2(Y299~P!1).PTPN6(PTP!1,Y566~P!?) -> DOK2(Y299~0) + PTPN6(PTP,Y566~P!?)

DOK2 Y299 0 1 ⬆P take one or more branches, once each, in any order⬆⬆ PTPN6 SH2 1 ⬆PTP Y566 P
Rule 34

WAS(PRS) + NCK(SH3_3) <-> WAS(PRS!1).NCK(SH3_3!1)

WAS 1 ⬆⬇PRS Y291 0 P NCK SH3_1 1 ⬆⬇SH3_3 SH2
Rule 35a

TCR(epitope!3,fynbind!1).FYN(unique!1).Lig3(aCD3!2,aCD3!3).TCR(epitope!2,PRS_E!4).NCK(SH3_1!4,SH3_3!5).WAS(Y291~0,PRS!5) -> TCR(epitope!3,fynbind!1).FYN(unique!1).Lig3(aCD3!2,aCD3!3).TCR(epitope!2,PRS_E!4).NCK(SH3_1!4,SH3_3!5).WAS(Y291~P,PRS!5)

TCR 3epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P 1fynbind PRS_E Y188_E 0 P Y199_E 0 P FYN 1unique PTK Lig3 2aCD3 3aCD3 TCR 2epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind 4PRS_E Y188_E 0 P Y199_E 0 P NCK 4SH3_1 5SH3_3 SH2 WAS 5PRS Y291 0 P take all branches, once each, in any order⬇⬇
Rule 35b

LAT(Y191~P!1).GRAP2(SH2!1,SH3!2).LCP2(RxxK!2,Y113_Y128~P!3).NCK(SH2!3,SH3_3!4).WAS(PRS!4,Y291~0) + FYN(unique!+,PTK) -> LAT(Y191~P!1).GRAP2(SH2!1,SH3!2).LCP2(RxxK!2,Y113_Y128~P!3).NCK(SH2!3,SH3_3!4).WAS(PRS!4,Y291~0!5).FYN(unique!+,PTK!5)

LAT Y132 0 P Y191 1P GRAP2 1SH2 2SH3 LCP2 2RxxK Y113_Y128 3P PRS Y145 0 P NCK SH3_1 4SH3_3 3SH2 WAS 4PRS Y291 5 ⬇0 FYN unique 5 ⬇PTK
Rule 35c

WAS(Y291~0!5).FYN(PTK!5) -> WAS(Y291~P) + FYN(PTK)

WAS PRS Y291 5 ⬆0 P take all branches, once each, in any order⬇⬇ FYN unique 5 ⬆PTK
Rule 35d

WAS(Y291~0!1).FYN(PTK!1) -> WAS(Y291~0) + FYN(PTK)

WAS PRS Y291 1 ⬆0 FYN unique 1 ⬆PTK
Rule 36a

ZAP70(SH2!+,PTK) + LAT(Y132~0) -> ZAP70(SH2!+,PTK!1).LAT(Y132~0!1)

ZAP70 SH2 1 ⬇PTK Y493 0 P LAT Y132 1 ⬇0 Y191 0 P
Rule 36b

ZAP70(PTK!1).LAT(Y132~0!1) -> ZAP70(PTK) + LAT(Y132~0)

ZAP70 SH2 1 ⬆PTK Y493 0 P LAT Y132 1 ⬆0 Y191 0 P
Rule 36c

ZAP70(PTK!1,Y493~P).LAT(Y132~0!1) -> ZAP70(PTK,Y493~P) + LAT(Y132~P)

ZAP70 SH2 1 ⬆PTK Y493 P LAT Y132 1 ⬆0 P take all branches, once each, in any order⬇⬇ Y191 0 P
Rule 37a

ZAP70(SH2!+,PTK) + LAT(Y191~0) -> ZAP70(SH2!+,PTK!1).LAT(Y191~0!1)

ZAP70 SH2 1 ⬇PTK Y493 0 P LAT Y132 0 P Y191 1 ⬇0
Rule 37b

ZAP70(PTK!1).LAT(Y191~0!1) -> ZAP70(PTK) + LAT(Y191~0)

ZAP70 SH2 1 ⬆PTK Y493 0 P LAT Y132 0 P Y191 1 ⬆0
Rule 37c

ZAP70(PTK!1,Y493~P).LAT(Y191~0!1) -> ZAP70(PTK,Y493~P) + LAT(Y191~P)

ZAP70 SH2 1 ⬆PTK Y493 P LAT Y132 0 P Y191 1 ⬆0 P take all branches, once each, in any order⬇⬇
Rule 38

PLCG1(SH2) + LAT(Y132~P) <-> PLCG1(SH2!1).LAT(Y132~P!1)

PLCG1 1 ⬆⬇SH2 SH3 Y783 0 P LAT Y132 1 ⬆⬇P Y191 0 P
Rule 39

LAT(Y191~P) + GRAP2(SH2) <-> LAT(Y191~P!1).GRAP2(SH2!1)

LAT Y132 0 P Y191 1 ⬆⬇P GRAP2 1 ⬆⬇SH2 SH3
Rule 40

GRAP2(SH3) + LCP2(RxxK) <-> GRAP2(SH3!1).LCP2(RxxK!1)

GRAP2 SH2 1 ⬆⬇SH3 LCP2 1 ⬆⬇RxxK Y113_Y128 0 P PRS Y145 0 P
Rule 41

NCK(SH2) + LCP2(Y113_Y128~P) <-> NCK(SH2!1).LCP2(Y113_Y128~P!1)

NCK SH3_1 SH3_3 1 ⬆⬇SH2 LCP2 RxxK Y113_Y128 1 ⬆⬇P PRS Y145 0 P
Rule 42a

LAT(Y191~P!1).GRAP2(SH2!1,SH3!2).LCP2(RxxK!2,Y113_Y128~0) + ZAP70(SH2!+,PTK) -> LAT(Y191~P!1).GRAP2(SH2!1,SH3!2).LCP2(RxxK!2,Y113_Y128~0!3).ZAP70(SH2!+,PTK!3)

LAT Y132 0 P Y191 1P GRAP2 1SH2 2SH3 LCP2 2RxxK Y113_Y128 3 ⬇0 PRS Y145 0 P ZAP70 SH2 3 ⬇PTK Y493 0 P
Rule 42b

ZAP70(PTK!1).LCP2(Y113_Y128~0!1) -> ZAP70(PTK) + LCP2(Y113_Y128~0)

ZAP70 SH2 1 ⬆PTK Y493 0 P LCP2 RxxK Y113_Y128 1 ⬆0 PRS Y145 0 P
Rule 42c

LCP2(Y113_Y128~0!3).ZAP70(PTK!3,Y493~P) -> LCP2(Y113_Y128~P) + ZAP70(PTK,Y493~P)

LCP2 RxxK Y113_Y128 3 ⬆0 P take all branches, once each, in any order⬇⬇ PRS Y145 0 P ZAP70 SH2 3 ⬆PTK Y493 P
Rule 43

PLCG1(SH3) + LCP2(PRS) <-> PLCG1(SH3!1).LCP2(PRS!1)

PLCG1 SH2 1 ⬆⬇SH3 Y783 0 P LCP2 RxxK Y113_Y128 0 P 1 ⬆⬇PRS Y145 0 P
Rule 44

LCP2(Y145~P) + ITK(SH2) <-> LCP2(Y145~P!1).ITK(SH2!1)

LCP2 RxxK Y113_Y128 0 P PRS Y145 1 ⬆⬇P ITK SH3 1 ⬆⬇SH2 PTK Y512 0 P
Rule 45a

LAT(Y191~P!1).GRAP2(SH2!1,SH3!2).LCP2(RxxK!2,Y145~0) + ZAP70(SH2!+,PTK) -> LAT(Y191~P!1).GRAP2(SH2!1,SH3!2).LCP2(RxxK!2,Y145~0!3).ZAP70(SH2!+,PTK!3)

LAT Y132 0 P Y191 1P GRAP2 1SH2 2SH3 LCP2 2RxxK Y113_Y128 0 P PRS Y145 3 ⬇0 ZAP70 SH2 3 ⬇PTK Y493 0 P
Rule 45b

ZAP70(PTK!1).LCP2(Y145~0!1) -> ZAP70(PTK) + LCP2(Y145~0)

ZAP70 SH2 1 ⬆PTK Y493 0 P LCP2 RxxK Y113_Y128 0 P PRS Y145 1 ⬆0
Rule 45c

LCP2(Y145~0!3).ZAP70(PTK!3,Y493~P) -> LCP2(Y145~P) + ZAP70(PTK,Y493~P)

LCP2 RxxK Y113_Y128 0 P PRS Y145 3 ⬆0 P take all branches, once each, in any order⬇⬇ ZAP70 SH2 3 ⬆PTK Y493 P
Rule 46

PLCG1(SH3!1,SH2!+,Y783~0).LCP2(PRS!1,Y145~P!2).ITK(SH2!2) -> PLCG1(SH3!1,SH2!+,Y783~P).LCP2(PRS!1,Y145~P!2).ITK(SH2!2)

PLCG1 SH2 1SH3 Y783 0 P take all branches, once each, in any order⬇⬇ LCP2 RxxK Y113_Y128 0 P 1PRS Y145 2P ITK SH3 2SH2 PTK Y512 0 P
The following rules account for phosphorylation and dephosphorylation in the basal state, and are not discussed in the model guide

TCR(Y149_D~0) -> TCR(Y149_D~P)

TCR epitope Y149_D 0 P take all branches, once each, in any order⬇⬇ Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y149_D~P) -> TCR(Y149_D~0)

TCR epitope Y149_D 0 P take one or more branches, once each, in any order⬆⬆ Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y171_G~0) -> TCR(Y171_G~P)

TCR epitope Y149_D 0 P Y171_G 0 P take all branches, once each, in any order⬇⬇ Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y171_G~P) -> TCR(Y171_G~0)

TCR epitope Y149_D 0 P Y171_G 0 P take one or more branches, once each, in any order⬆⬆ Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y111~0) -> TCR(Y111~P)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P take all branches, once each, in any order⬇⬇ Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y111~P) -> TCR(Y111~0)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P take one or more branches, once each, in any order⬆⬆ Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y123~0) -> TCR(Y123~P)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P take all branches, once each, in any order⬇⬇ fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y123~P) -> TCR(Y123~0)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P take one or more branches, once each, in any order⬆⬆ fynbind PRS_E Y188_E 0 P Y199_E 0 P

TCR(Y199_E~0) -> TCR(Y199_E~P)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P take all branches, once each, in any order⬇⬇

TCR(Y199_E~P) -> TCR(Y199_E~0)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P Y199_E 0 P take one or more branches, once each, in any order⬆⬆

TCR(Y188_E~0) -> TCR(Y188_E~P)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P take all branches, once each, in any order⬇⬇ Y199_E 0 P

TCR(Y188_E~P) -> TCR(Y188_E~0)

TCR epitope Y149_D 0 P Y171_G 0 P Y111 0 P Y123 0 P fynbind PRS_E Y188_E 0 P take one or more branches, once each, in any order⬆⬆ Y199_E 0 P

LCK(Y192~0) -> LCK(Y192~P)

LCK SH2 SH3 Y192 0 P take all branches, once each, in any order⬇⬇ Y424 0 P Y505 0 P

LCK(Y192~P) -> LCK(Y192~0)

LCK SH2 SH3 Y192 0 P take one or more branches, once each, in any order⬆⬆ Y424 0 P Y505 0 P

LCK(Y424~0) -> LCK(Y424~P)

LCK SH2 SH3 Y192 0 P Y424 0 P take all branches, once each, in any order⬇⬇ Y505 0 P

LCK(Y424~P) -> LCK(Y424~0)

LCK SH2 SH3 Y192 0 P Y424 0 P take one or more branches, once each, in any order⬆⬆ Y505 0 P

LCK(Y505~0) -> LCK(Y505~P)

LCK SH2 SH3 Y192 0 P Y424 0 P Y505 0 P take all branches, once each, in any order⬇⬇

LCK(Y505~P) -> LCK(Y505~0)

LCK SH2 SH3 Y192 0 P Y424 0 P Y505 0 P take one or more branches, once each, in any order⬆⬆

PTPN6(Y566~0) -> PTPN6(Y566~P)

PTPN6 SH2 PTP Y566 0 P take all branches, once each, in any order⬇⬇

PTPN6(Y566~P) -> PTPN6(Y566~0)

PTPN6 SH2 PTP Y566 0 P take one or more branches, once each, in any order⬆⬆

ZAP70(Y493~0) -> ZAP70(Y493~P)

ZAP70 SH2 PTK Y493 0 P take all branches, once each, in any order⬇⬇

ZAP70(Y493~P) -> ZAP70(Y493~0)

ZAP70 SH2 PTK Y493 0 P take one or more branches, once each, in any order⬆⬆

LAT(Y132~0) -> LAT(Y132~P)

LAT Y132 0 P take all branches, once each, in any order⬇⬇ Y191 0 P

LAT(Y132~P) -> LAT(Y132~0)

LAT Y132 0 P take one or more branches, once each, in any order⬆⬆ Y191 0 P

LAT(Y191~0) -> LAT(Y191~P)

LAT Y132 0 P Y191 0 P take all branches, once each, in any order⬇⬇

LAT(Y191~P) -> LAT(Y191~0)

LAT Y132 0 P Y191 0 P take one or more branches, once each, in any order⬆⬆

ITK(Y512~0) -> ITK(Y512~P)

ITK SH3 SH2 PTK Y512 0 P take all branches, once each, in any order⬇⬇

ITK(Y512~P) -> ITK(Y512~0)

ITK SH3 SH2 PTK Y512 0 P take one or more branches, once each, in any order⬆⬆

PLCG1(Y783~0) -> PLCG1(Y783~P)

PLCG1 SH2 SH3 Y783 0 P take all branches, once each, in any order⬇⬇

PLCG1(Y783~P) -> PLCG1(Y783~0)

PLCG1 SH2 SH3 Y783 0 P take one or more branches, once each, in any order⬆⬆

LCP2(Y113_Y128~0) -> LCP2(Y113_Y128~P)

LCP2 RxxK Y113_Y128 0 P take all branches, once each, in any order⬇⬇ PRS Y145 0 P

LCP2(Y113_Y128~P) -> LCP2(Y113_Y128~0)

LCP2 RxxK Y113_Y128 0 P take one or more branches, once each, in any order⬆⬆ PRS Y145 0 P

LCP2(Y145~0) -> LCP2(Y145~P)

LCP2 RxxK Y113_Y128 0 P PRS Y145 0 P take all branches, once each, in any order⬇⬇

LCP2(Y145~P) -> LCP2(Y145~0)

LCP2 RxxK Y113_Y128 0 P PRS Y145 0 P take one or more branches, once each, in any order⬆⬆

PAG1(Y163~0) -> PAG1(Y163~P)

PAG1 Y163 0 P take all branches, once each, in any order⬇⬇ Y317 0 P

PAG1(Y163~P) -> PAG1(Y163~0)

PAG1 Y163 0 P take one or more branches, once each, in any order⬆⬆ Y317 0 P

PAG1(Y317~0) -> PAG1(Y317~P)

PAG1 Y163 0 P Y317 0 P take all branches, once each, in any order⬇⬇

PAG1(Y317~P) -> PAG1(Y317~0)

PAG1 Y163 0 P Y317 0 P take one or more branches, once each, in any order⬆⬆

DOK1(Y449~0) -> DOK1(Y449~P)

DOK1 Y449 0 P take all branches, once each, in any order⬇⬇

DOK1(Y449~P) -> DOK1(Y449~0)

DOK1 Y449 0 P take one or more branches, once each, in any order⬆⬆

DOK2(Y299~0) -> DOK2(Y299~P)

DOK2 Y299 0 P take all branches, once each, in any order⬇⬇

DOK2(Y299~P) -> DOK2(Y299~0)

DOK2 Y299 0 P take one or more branches, once each, in any order⬆⬆

WAS(Y291~0) -> WAS(Y291~P)

WAS PRS Y291 0 P take all branches, once each, in any order⬇⬇

WAS(Y291~P) -> WAS(Y291~0)

WAS PRS Y291 0 P take one or more branches, once each, in any order⬆⬆