Molecules

R(DD,Y1~U~P,Y2~P)

R DD Y1 U P Y2 P

S(NSH2~C~O,CSH2,PTP~C~O)

S NSH2 C O CSH2 PTP C O

Species

S(NSH2~C,CSH2,PTP~C)

S NSH2 C CSH2 PTP C
Pre-dimerized receptors

R(DD!1,Y1~U,Y2~P).R(DD!1,Y1~U,Y2~P)

R 1DD Y1 U Y2 P R 1DD Y1 U Y2 P

Observables

R(Y1~P!?)

R DD Y1 P Y2 P

Reaction Rules

Intra-complex phosphorylation

R(DD!+,Y1~U) -> R(DD!+,Y1~P)

R DD Y1 U P take all branches, once each, in any order⬇⬇ Y2 P
Equilibrium between the closed form and open form of S

S(NSH2~C,PTP~C) <-> S(NSH2~O,PTP~O)

S NSH2 C O take all branches, once each, in any order⬇⬇ CSH2 PTP C O take all branches, once each, in any order⬇⬇
Binding of S(CSH2) from cytosol

R(Y2~P) + S(CSH2) <-> R(Y2~P!1).S(CSH2!1) kon_CSH2,koff_CSH2 exclude_reactants(2,R)

R DD Y1 U P Y2 1 ⬆⬇P S NSH2 C O 1 ⬆⬇CSH2 PTP C O
Binding of S(NSH2~O) from cytosol

R(Y2~P) + S(NSH2~O) <-> R(Y2~P!1).S(NSH2~O!1) kon_NSH2,koff_NSH2 exclude_reactants(2,R)

R DD Y1 U P Y2 1 ⬆⬇P S NSH2 1 ⬆⬇O CSH2 PTP C O
Binding of S(PTP~O) from cytosol

R(Y1~P) + S(PTP~O) <-> R(Y1~P!1).S(PTP~O!1) kon_PTP,koff_PTP exclude_reactants(2,R)

R DD Y1 1 ⬆⬇P Y2 P S NSH2 C O CSH2 PTP 1 ⬆⬇O
Dephosphorylation of R(Y1~P)

R(Y1~P!1).S(PTP~O!1) -> R(Y1~U) + S(PTP~O)

R DD Y1 U 1 ⬆P take one or more branches, once each, in any order⬆⬆ Y2 P S NSH2 C O CSH2 PTP 1 ⬆O

R(Y1~P!1).S(PTP~O!1) -> R(Y1~U).S(PTP~O)

R DD Y1 U 1 ⬆P take one or more branches, once each, in any order⬆⬆ Y2 P S NSH2 C O CSH2 PTP 1 ⬆O
1 Intra-complex binding: CSH2 bound, association of NSH2 (open) with other receptor

R(Y2~P).S(NSH2~O,CSH2!+,PTP~O) <-> R(Y2~P!1).S(NSH2~O!1,CSH2!+,PTP~O)

R DD Y1 U P Y2 1 ⬆⬇P S NSH2 1 ⬆⬇O CSH2 PTP O
2 Intra-complex binding: CSH2 bound, association of PTP (open) with same receptor

R(Y1~P,Y2~P!1).S(NSH2~O,CSH2!1,PTP~O) <-> R(Y1~P!2,Y2~P!1).S(NSH2~O,CSH2!1,PTP~O!2)

R DD Y1 2 ⬆⬇P Y2 1P S NSH2 O 1CSH2 PTP 2 ⬆⬇O
3 Intra-complex binding: CSH2 bound, association of PTP (open) with other receptor

R(Y1~P).R(Y2~P!1).S(NSH2~O,CSH2!1,PTP~O) <-> R(Y1~P!2).R(Y2~P!1).S(NSH2~O,CSH2!1,PTP~O!2)

R DD Y1 2 ⬆⬇P Y2 P R DD Y1 U P Y2 1P S NSH2 O 1CSH2 PTP 2 ⬆⬇O
4 Intra-complex binding: NSH2 bound, association of CSH2 with other receptor

R(Y2~P).S(NSH2~O!+,CSH2,PTP~O) <-> R(Y2~P!1).S(NSH2~O!+,CSH2!1,PTP~O)

R DD Y1 U P Y2 1 ⬆⬇P S NSH2 O 1 ⬆⬇CSH2 PTP O
5 Intra-complex binding: NSH2 bound, association of PTP with other receptor

R(Y1~P).R(Y2~P!1).S(NSH2~O!1,CSH2,PTP~O) <-> R(Y1~P!2).R(Y2~P!1).S(NSH2~O!1,CSH2,PTP~O!2)

R DD Y1 2 ⬆⬇P Y2 P R DD Y1 U P Y2 1P S NSH2 1O CSH2 PTP 2 ⬆⬇O
6 Intracomplex binding: NSH2 bound, association of PTP with same receptor

R(Y1~P,Y2~P!1).S(NSH2~O!1,CSH2,PTP~O) <-> R(Y1~P!2,Y2~P!1).S(NSH2~O!1,CSH2,PTP~O!2)

R DD Y1 2 ⬆⬇P Y2 1P S NSH2 1O CSH2 PTP 2 ⬆⬇O
7 Intra-complex binding: PTP bound, association of CSH2 with same receptor

R(Y1~P!1,Y2~P).S(NSH2~O,CSH2,PTP~O!1) <-> R(Y1~P!1,Y2~P!2).S(NSH2~O,CSH2!2,PTP~O!1)

R DD Y1 1P Y2 2 ⬆⬇P S NSH2 O 2 ⬆⬇CSH2 PTP 1O
8 Intra-complex binding: PTP bound, association of CSH2 with other receptor

R(Y1~P!1).R(Y2~P).S(NSH2~O,CSH2,PTP~O!1) <-> R(Y1~P!1).R(Y2~P!2).S(NSH2~O,CSH2!2,PTP~O!1)

R DD Y1 1P Y2 P R DD Y1 U P Y2 2 ⬆⬇P S NSH2 O 2 ⬆⬇CSH2 PTP 1O
9 Intra-complex binding: PTP bound, association of NSH2 with other receptor

R(Y1~P!1).R(Y2~P).S(NSH2~O,CSH2,PTP~O!1) <-> R(Y1~P!1).R(Y2~P!2).S(NSH2~O!2,CSH2,PTP~O!1)

R DD Y1 1P Y2 P R DD Y1 U P Y2 2 ⬆⬇P S NSH2 2 ⬆⬇O CSH2 PTP 1O
10 Intra-complex binding: PTP bound, association of NSH2 with same receptor

R(Y1~P!1,Y2~P).S(NSH2~O,CSH2,PTP~O!1) <-> R(Y1~P!1,Y2~P!2).S(NSH2~O!2,CSH2,PTP~O!1)

R DD Y1 1P Y2 2 ⬆⬇P S NSH2 2 ⬆⬇O CSH2 PTP 1O
11 Intra-complex binding: CSH2 & NSH2 bound, assoc. of PTP with same receptor as CSH2

R(Y1~P,Y2~P!1).R(Y2~P!2).S(NSH2~O!2,CSH2!1,PTP~O) <-> R(Y1~P!3,Y2~P!1).R(Y2~P!2).S(NSH2~O!2,CSH2!1,PTP~O!3)

R DD Y1 3 ⬆⬇P Y2 1P R DD Y1 U P Y2 2P S NSH2 2O 1CSH2 PTP 3 ⬆⬇O
12 Intra-complex binding: CSH2 & NSH2 bound, assoc. of PTP with same receptor as NSH2

R(Y1~P,Y2~P!1).R(Y2~P!2).S(NSH2~O!1,CSH2!2,PTP~O) <-> R(Y1~P!3,Y2~P!1).R(Y2~P!2).S(NSH2~O!1,CSH2!2,PTP~O!3)

R DD Y1 3 ⬆⬇P Y2 1P R DD Y1 U P Y2 2P S NSH2 1O 2CSH2 PTP 3 ⬆⬇O
13 Intra-complex binding: CSH2 & PTP bound to the same receptor, assoc. of NSH2

R(Y1~P!1,Y2~P!2).R(Y2~P).S(NSH2~O,CSH2!2,PTP~O!1) <-> R(Y1~P!1,Y2~P!2).R(Y2~P!3).S(NSH2~O!3,CSH2!2,PTP~O!1)

R DD Y1 1P Y2 2P R DD Y1 U P Y2 3 ⬆⬇P S NSH2 3 ⬆⬇O 2CSH2 PTP 1O
14 Intra-complex binding: CSH2 & PTP bound to different receptors, assoc. of NSH2

R(Y2~P!1).R(Y1~P!2,Y2~P).S(NSH2~O,CSH2!1,PTP~O!2) <-> R(Y2~P!1).R(Y1~P!2,Y2~P!3).S(NSH2~O!3,CSH2!1,PTP~O!2)

R DD Y1 U P Y2 1P R DD Y1 2P Y2 3 ⬆⬇P S NSH2 3 ⬆⬇O 1CSH2 PTP 2O
15 Intra-complex binding: PTP & NSH2 bound to different receptors, assoc. of CSH2

R(Y2~P!1).R(Y1~P!2,Y2~P).S(NSH2~O!1,CSH2,PTP~O!2) <-> R(Y2~P!1).R(Y1~P!2,Y2~P!3).S(NSH2~O!1,CSH2!3,PTP~O!2)

R DD Y1 U P Y2 1P R DD Y1 2P Y2 3 ⬆⬇P S NSH2 1O 3 ⬆⬇CSH2 PTP 2O
16 Intra-complex binding: PTP & NSH2 bound to same receptor, assoc. of CSH2

R(Y1~P!1,Y2~P!2).R(Y2~P).S(NSH2~O!2,CSH2,PTP~O!1) <-> R(Y1~P!1,Y2~P!2).R(Y2~P!3).S(NSH2~O!2,CSH2!3,PTP~O!1)

R DD Y1 1P Y2 2P R DD Y1 U P Y2 3 ⬆⬇P S NSH2 2O 3 ⬆⬇CSH2 PTP 1O